identification of campylobacter spp. from poultry skin using methods based on bacterial culture and polymerase chain reactions

نویسندگان

فاطمه ابراهیمی لقا

دانش آموخته صنایع غذایی، دانشگاه ارومیه، ارومیه، ایران فریبا زینالی

گروه صنایع غذایی، دانشکده کشاورزی دانشگاه ارومیه، ارومیه، ایران محمود رضازاد باری

گروه صنایع غذایی، دانشکده کشاورزی دانشگاه ارومیه، ارومیه، ایران

چکیده

background: food infections caused by campylobacter are one of the gastrointestinal inflammations in humans is health and economic losses in the community is important. objectives: to determine the prevalence of campylobacter contamination in chicken skin samples of urmia, using bacterial culture and polymerase chain reactions.                 methods: 80 samples of chicken skin from the protein gostare sina slaughter house located in the city of urmia in equal numbers in the winter and spring seasons were collected. the survival of campylobacter after 24 hours in refrigerated conditions  was studied in samples. positive samples were used for dna extraction and pcr. to investigate the phylogenetic isolates, positive samples pcr were sequenced. results: 58/75% of chicken skin using bacterial cultures, campylobacter were positive. the results study the survival campylobacter in cold conditions after 24 hours, showed that no significant decrease in the survival campylobacter as well as contamination levels were significantly higher in spring than in winter, which may be due to the high temperature of environment that created the favorable conditions for campylobacter. conclusions: chicken skin is the reservoir  of campylobacter. this issue of public health care and control at all stages of production and supply of poultry products, also the transfer of it to other parts of poultry carcasses should be considered.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and identification of Campylobacter jejuni from poultry carcasses using conventional culture methods and multiplex PCR assay

Campylobacter jejuni is a major cause of food-borne diarrhea in many countries. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, conventional culture and multiplex PCR methods were compared for the detection of C. jejuni isolated from poultry carcasses. A total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...

متن کامل

Isolation and identification of Campylobacter spp. and Campylobacter coli from poultry carcasses by conventional culture method and multiplex PCR in Mashhad, Iran

The genus Campylobacter  is of great importance to public health because it includes several species that may cause diarrhoea. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, during the autumn months of 2005, a total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage ...

متن کامل

Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

متن کامل

isolation and identification of campylobacter jejuni from poultry carcasses using conventional culture methods and multiplex pcr assay

campylobacter jejuni is a major cause of food-borne diarrhea in many countries. poultry and poultry products are known as important sources of human campylobacteriosis. in this study, conventional culture and multiplex pcr methods were compared for the detection of c. jejuni isolated from poultry carcasses. a total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...

متن کامل

Direct polymerase chain reaction detection of Campylobacter spp. in poultry hatchery samples.

A rapid, sensitive, and specific polymerase chain reaction (PCR) assay was developed for the direct detection of Campylobacter in environmental samples from hatcheries. PCR, with a set of primers specific for the Campylobacter flaA short variable region (SVR), detected the presence of Campylobacter in both fluff and eggshell samples; however, a determination of whether the organism was living o...

متن کامل

Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods.

AIMS To investigate the optimal method for the detection of campylobacters from stool samples by comparing selective culture with membrane filtration and the polymerase chain reaction (PCR). METHODS Three hundred and forty three stool samples were investigated by each of the three methods mentioned above. Selective culture was performed with charcoal cefoperazone desoxycholate agar plates. Me...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
تحقیقات دامپزشکی

جلد ۷۱، شماره ۴، صفحات ۴۳۱-۴۳۶

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023